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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Traffic. 2013 Mar 20;14(6):650–662. doi: 10.1111/tra.12064

Figure 3. Non-canonical SNARE complexes support fusion when their surface density is increased.

Figure 3

(A) Standard fusion reactions containing vacuoles from wild type strains (black bars) or SNARE overexpressing strains (gray bars) were blocked at the priming stage with 85 μg/ml anti-Sec17p antibody for 15 minutes. Recombinant GST-Vam7p (400 nM, black bars) or Vam7pQ283R (gray bars) was added to bypass the priming block. Error bars represent SEM (n=3). (B) GST-Vam7p protein complexes were isolated as described in the Materials and Methods section and examined by immunoblotting for the indicated proteins.