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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Traffic. 2013 Mar 20;14(6):650–662. doi: 10.1111/tra.12064

Figure 5. CPZ and Vam7p bypass anti-Sec17p IgG-blocked fusion of vacuoles containing lipid-anchored Vam3p.

Figure 5

(A) Vacuoles from wild type DKY6281 were incubated with BJ3505 vacuoles harboring Vam3p-CCIIM. Priming was blocked with anti-Sec17p antibody for 15 minutes and reactions were treated with 150 μM CPZ for 5 minutes before the addition of 400 nM Vam7p or Vam7pQ283R. (B) Fusion reactions were performed with DKY6281 and BJ3505 vacuoles harboring Vam3p-CCIIM. Fusion reactions were treated with CPZ and Vam7p as described in (A). Error bars represent SEM (n=3).