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. 1972 May;49(5):783–788. doi: 10.1104/pp.49.5.783

Characterization of Short Time Labeled Adenosine Monophosphate-rich Ribonucleic Acids of Soybean 1

Joe L Key a, C J Leaver a,2, J R Cowles a,3, J M Anderson a
PMCID: PMC366052  PMID: 16658048

Abstract

The total population of newly synthesized 32P-AMP-rich RNA has been separated into two major types based on repeated fractionation on methylated albumin-kieselguhr columns. The purified D-RNA which elutes, under our experimental conditions, primarily in the salt gradient has a GMP/AMP ratio of about 0.8 and an AMP + UMP content of about 56 mole per cent. The purified TB-RNA which preferentially remains bound to the column in the salt gradient has a GMP/AMP ratio of about 0.4 to 0.45 and an AMP + UMP content of about 65 mole per cent. In addition to being distinguished by their fractionation on the methylated albumin-kieselguhr column and base composition analysis, purified D-RNA and TB-RNA have different size distributions on sucrose gradient and acrylamide gel fractionation, are differentially associated with polyribosomes and have different stabilities in the tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Huang A. S. Interaction of HeLa cell proteins with RNA. J Mol Biol. 1970 Feb 14;47(3):263–273. doi: 10.1016/0022-2836(70)90301-3. [DOI] [PubMed] [Google Scholar]
  2. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chroboczek H., Cherry J. H. Characterization of nucleic acids in peanut cotyledons. J Mol Biol. 1966 Aug;19(1):28–37. doi: 10.1016/s0022-2836(66)80047-5. [DOI] [PubMed] [Google Scholar]
  4. Darnell J. E., Jr Ribonucleic acids from animal cells. Bacteriol Rev. 1968 Sep;32(3):262–290. doi: 10.1128/br.32.3.262-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Darnell J. E., Wall R., Tushinski R. J. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. doi: 10.1073/pnas.68.6.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ellem K. A., Sheridan J. W. Tenacious binding of the bulk of the DNA-like RNA of metazoan cells to methylated albumin columns. Biochem Biophys Res Commun. 1964 Aug 11;16(6):505–510. doi: 10.1016/0006-291x(64)90183-4. [DOI] [PubMed] [Google Scholar]
  8. Ellem K. A. Some properties of mammalian DNA-like RNA isolated by chromatography on methylated bovine serum albumin-kieselguhr columns. J Mol Biol. 1966 Sep;20(2):283–305. doi: 10.1016/0022-2836(66)90065-9. [DOI] [PubMed] [Google Scholar]
  9. Hadjivassiliou A., Brawerman G. Template and ribosomal ribonucleic acid components in the nucleus and the cytoplasm of rat liver. Biochemistry. 1967 Jul;6(7):1934–1941. doi: 10.1021/bi00859a008. [DOI] [PubMed] [Google Scholar]
  10. Henshaw E. C., Loebenstein J. Rapidly labeled, polydisperse RNA in rat-liver cytoplasm: evidence that it is contained in ribonucleoprotein particles of heterogeneous size. Biochim Biophys Acta. 1970 Feb 18;199(2):405–420. doi: 10.1016/0005-2787(70)90083-3. [DOI] [PubMed] [Google Scholar]
  11. Henshaw E. C. Messenger RNA in rat liver polyribosomes: evidence that it exists as ribonucleoprotein particles. J Mol Biol. 1968 Sep 28;36(3):401–411. doi: 10.1016/0022-2836(68)90164-2. [DOI] [PubMed] [Google Scholar]
  12. INGLE J., KEY J. L., HOLM R. E. DEMONSTRATION AND CHARACTERIZATION OF A DNA-LIKE RNA IN EXCISED PLANT TISSUE. J Mol Biol. 1965 Apr;11:730–746. doi: 10.1016/s0022-2836(65)80031-6. [DOI] [PubMed] [Google Scholar]
  13. Infante A. A., Nemer M. Heterogeneous ribonucleoprotein particles in the cytoplasm of sea urchin embryos. J Mol Biol. 1968 Mar 28;32(3):543–565. doi: 10.1016/0022-2836(68)90342-2. [DOI] [PubMed] [Google Scholar]
  14. Ingle J., Key J. L. A re-evaluation of the fractionation of high molecular weight RNA by MAK chromatography. Biochem Biophys Res Commun. 1968 Mar 27;30(6):711–716. doi: 10.1016/0006-291x(68)90571-8. [DOI] [PubMed] [Google Scholar]
  15. Johri M. M., Varner J. E. Characterization of rapidly labeled ribonucleic acid from dwarf peas. Plant Physiol. 1970 Mar;45(3):348–357. doi: 10.1104/pp.45.3.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Key J. L. Effect of purine and pyrimidine analogues on growth and RNA metabolism in the soybean hypocotyl-the selective action of 5-fluorouracil. Plant Physiol. 1966 Oct;41(8):1257–1264. doi: 10.1104/pp.41.8.1257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Key J. L., Ingle J. REQUIREMENT FOR THE SYNTHESIS OF DNA-LIKE RNA FOR GROWTH OF EXCISED PLANT TISSUE. Proc Natl Acad Sci U S A. 1964 Dec;52(6):1382–1388. doi: 10.1073/pnas.52.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Leaver C. J., Key J. L. Polyribosome formation and RNA synthesis during aging of carrot-root tissue. Proc Natl Acad Sci U S A. 1967 May;57(5):1338–1344. doi: 10.1073/pnas.57.5.1338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Leaver C. J., Key J. L. Ribosomal RNA synthesis in plants. J Mol Biol. 1970 May 14;49(3):671–680. doi: 10.1016/0022-2836(70)90290-1. [DOI] [PubMed] [Google Scholar]
  20. Lee S. Y., Brawerman G. Pulse-labeled ribonucleic acid complexes released by dissociation of rat liver polysomes. Biochemistry. 1971 Feb 2;10(3):510–516. doi: 10.1021/bi00779a025. [DOI] [PubMed] [Google Scholar]
  21. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lim L., Canellakis E. S. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970 Aug 15;227(5259):710–712. doi: 10.1038/227710a0. [DOI] [PubMed] [Google Scholar]
  23. Lim L., Canellakis Z. N., Canellakis E. S. Metabolism of naturally occurring homopolymers. II. Characterization of adenine-rich polynucleotides of mouse liver ribosomes. Biochim Biophys Acta. 1970 May 21;209(1):128–138. [PubMed] [Google Scholar]
  24. Lin C. Y., Key J. L., Bracker C. E. Association of D-RNA with Polyribosomes in the Soybean Root. Plant Physiol. 1966 Jun;41(6):976–982. doi: 10.1104/pp.41.6.976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Loening U. E. Synthesis of messenger ribonucleic acid in excised pea-seedling root segments. Separation of the messenger from microsomes by electrophoresis. Biochem J. 1965 Oct;97(1):125–133. doi: 10.1042/bj0970125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  28. Olsnes S. Characterization of protein bound to rapidly-labelled RNA in polyribosomes from rat liver. Eur J Biochem. 1970 Sep;15(3):464–471. doi: 10.1111/j.1432-1033.1970.tb01029.x. [DOI] [PubMed] [Google Scholar]
  29. Perry R. P., Kelley D. E. Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes. J Mol Biol. 1968 Jul 14;35(1):37–59. doi: 10.1016/s0022-2836(68)80035-x. [DOI] [PubMed] [Google Scholar]
  30. Rogers M. E., Loening U. E., Fraser R. S. Ribosomal RNA precursors in plants. J Mol Biol. 1970 May 14;49(3):681–692. doi: 10.1016/0022-2836(70)90291-3. [DOI] [PubMed] [Google Scholar]
  31. Shearer R. W., McCarthy B. J. Evidence for ribonucleic acid molecules restricted to the cell nucleus. Biochemistry. 1967 Jan;6(1):283–289. doi: 10.1021/bi00853a044. [DOI] [PubMed] [Google Scholar]
  32. Soeiro R., Vaughan M. H., Warner J. R., Darnell J. E., Jr The turnover of nuclear DNA-like RNA in HeLa cells. J Cell Biol. 1968 Oct;39(1):112–118. doi: 10.1083/jcb.39.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sussman M. Model for quantitative and qualitative control of mRNA translation in eukaryotes. Nature. 1970 Mar 28;225(5239):1245–1246. doi: 10.1038/2251245a0. [DOI] [PubMed] [Google Scholar]
  34. Tester C. F., Dure L. S., 3rd Nucleic acid synthesis during the hormone-stimulated growth of excised oat coleoptiles. Biochemistry. 1967 Aug;6(8):2532–2538. doi: 10.1021/bi00860a034. [DOI] [PubMed] [Google Scholar]

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