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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Phytomedicine. 2013 Apr 1;20(0):676–682. doi: 10.1016/j.phymed.2013.02.008

Fig. 1. Effect of DPHD on osteoblast mRNAs at seven days.

Fig. 1

A. Structures of (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (DPHD) and 17β-estradiol (E2).

B. Expression of estrogen receptors (ER) α and β, RUNX2 and OPG mRNA in total RNA from h-OB not treated (open bars), treated with E2 (0.010 μM, gray bars) or treated with DPHD (0.10 or 1.0 μM, filled bars), with and without the ER antagonist ICI182780 (1 μM) for 7 days. The qPCR data were normalized to GAPDH. Each value is Mean±SEM from 3 independent experiments; **p < 0.01 relative to controls (open bars); #p < 0.05 relative to addition of ICI187280.