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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Phytomedicine. 2013 Apr 1;20(0):676–682. doi: 10.1016/j.phymed.2013.02.008

Fig. 2. Effect of DPHD on cell proliferation and on Erk1/2 kinase.

Fig. 2

A. Expression of phospho-Erk1/2 were shown at 30 minutes (left) and at 60 minutes (right). Reprobing for total Erk1/2 and β-actin show equivalent Erk and total protein. The bar graphs (bottom) show densitometry for p-Erk/Erk.

B. Human osteoblast precursor cells in growth medium containing compounds for 24 hours (open bars) and 72 hours (filled bars), evaluated using the MTT assay.

C. Thymidine incorporation at 24 hours. n=4.

D. To confirm estrogen-receptor dependence, MTT assays at 24 hours were done using ICI182780 without treatment (open bars) and with 10 nM E2 (gray bars) or 0.1–1 μM DPHD (filled bars). Each value is Mean±SEM from 3 independent experiments, otherwise indicated; *p < 0.05 or **p < 0.01 relative to controls.