Table 4. Expression levels of virulence-related genes in biofilms of C. albicans SC5314 (WT) grown on SB discs in the presence or absence of human serum.
| (A) | (B) | (C) | ||||||||||||||||
| 90 min | 24 h | 48 h | ||||||||||||||||
| YNB | YNB+3% human serum | YNB | YNB+3% human serum | YNB | YNB+3% human serum | |||||||||||||
| Mean | ± SD | Mean | ± SD | p-value | Mean | ± SD | Mean | ± SD | p-value | Mean | ± SD | Mean | ± SD | p-value | ||||
| ALS3 | 2.45 | (0.190) | 1.92 | (0.297) | ns | ↓ | 1.63 | (0.119) | 2.01 | (0.282) | ns | ↑ | 0.70 | (0.035) | 0.90 | (0.012) | ** | ↑ |
| ECE1 | 11.84 | (1.025) | 20.08 | (2.551) | ** | ↑ | 2.23 | (0.483) | 5.26 | (0.327) | *** | ↑ | 2.56 | (0.312) | 3.18 | (0.160) | * | ↑ |
| EAP1 | 0.36 | (0.017) | 0.31 | (0.015) | * | ↓ | 0.72 | (0.088) | 0.90 | (0.034) | * | ↑ | 0.73 | (0.051) | 0.95 | (0.168) | ns | ↑ |
| HWP1 | 16.83 | (1.151) | 13.11 | (0.031) | ** | ↓ | 1.10 | (0.233) | 1.04 | (0.130) | ns | ↓ | 0.52 | (0.042) | 0.92 | (0.178) | * | ↑ |
| SAP1 | 0.10 | (0.006) | 0.15 | (0.005) | *** | ↑ | 0.49 | (0.012) | 0.76 | (0.003) | *** | ↑ | 0.86 | (0.053) | 1.62 | (0.433) | * | ↑ |
| SAP2 | 0.31 | (0.004) | 0.23 | (0.021) | ** | ↓ | 1.01 | (0.028) | 1.59 | (0.129) | ** | ↑ | 1.43 | (0.165) | 1.82 | (0.175) | * | ↑ |
| SAP3 | 0.17 | (0.037) | 0.22 | (0.023) | ns | ↑ | 0.79 | (0.185) | 1.00 | (0.103) | ns | ↑ | 0.76 | (0.073) | 1.02 | (0.184) | ns | ↑ |
| SAP4 | 0.09 | (0.012) | 0.21 | (0.039) | ** | ↑ | 0.54 | (0.023) | 1.03 | (0.033) | *** | ↑ | 0.97 | (0.124) | 1.56 | (0.019) | ** | ↑ |
| SAP5 | 0.11 | (0.021) | 0.14 | (0.007) | ns | ↑ | 0.59 | (0.047) | 0.72 | (0.035) | * | ↑ | 0.75 | (0.087) | 0.88 | (0.085) | ns | ↑ |
| SAP6 | 0.12 | (0.004) | 0.18 | (0.018) | ** | ↑ | 0.58 | (0.081) | 0.81 | (0.082) | * | ↑ | 0.80 | (0.061) | 1.21 | (0.021) | *** | ↑ |
| SAP7 | 0.56 | (0.039) | 0.40 | (0.064) | * | ↓ | 0.52 | (0.182) | 0.61 | (0.041) | ns | ↑ | 0.54 | (0.123) | 0.81 | (0.133) | ns | ↑ |
| SAP8 | 0.21 | (0.016) | 0.39 | (0.064) | * | ↑ | 0.74 | (0.035) | 1.14 | (0.071) | ** | ↑ | 1.01 | (0.199) | 1.78 | (0.230) | * | ↑ |
| SAP9 | 0.20 | (0.019) | 0.25 | (0.018) | * | ↑ | 0.62 | (0.013) | 0.67 | (0.017) | * | ↑ | 1.31 | (0.088) | 1.62 | (0.134) | * | ↑ |
| SAP10 | 0.45 | (0.016) | 0.56 | (0.069) | * | ↑ | 0.09 | (0.011) | 0.71 | (0.091) | *** | ↑ | 1.39 | (0.125) | 2.01 | (0.159) | ** | ↑ |
| PLB1 | 0.18 | (0.061) | 0.17 | (0.012) | ns | ↓ | 0.69 | (0.064) | 0.90 | (0.077) | * | ↑ | 0.68 | (0.090) | 0.77 | (0.148) | ns | ↑ |
| PLB2 | 0.06 | (0.008) | 0.08 | (0.002) | * | ↑ | 0.27 | (0.051) | 0.35 | (0.035) | ns | ↑ | 0.40 | (0.035) | 0.47 | (0.081) | ns | ↑ |
| PLC | 0.19 | (0.006) | 0.25 | (0.010) | ** | ↑ | 0.59 | (0.044) | 0.54 | (0.032) | ns | ↓ | 0.72 | (0.020) | 0.62 | (0.098) | ns | ↓ |
| PLD | 0.38 | (0.036) | 0.44 | (0.055) | ns | ↑ | 0.82 | (0.237) | 1.18 | (0.223) | ns | ↑ | 0.81 | (0.043) | 1.39 | (0.178) | ** | ↑ |
Samples were taken during the adhesion phase (90 min) and biofilm growth (24 h and 48 h). Results are the mean ± SD values of three independent experiments.
ns : p>0.05;
*
p<0.05;
**
p<0.01;
***
p<0.001.
The arrows refer to up/down regulation of mRNA expression levels of virulence genes of C. albicans SC5314 (WT) in the presence of human serum relative to the mRNA expression levels devoid of serum.