Skip to main content
. 2013 May 21;8(5):e63896. doi: 10.1371/journal.pone.0063896

Figure 7. RLX and Notch-1 negatively regulates TGF-β1 signaling in primary neonatal cardiac fibroblasts.

Figure 7

(A) Western blotting analysis of Smad3 expression and (B) phosphorylation (performed on the immunoprecipitated Smad3 proteins) in cardiac fibroblasts cultured for 48 h in the indicated experimental conditions. Data are representative of at least three independent experiments. Histograms show the densitometric analyses of the bands normalized to (A) GAPDH and (B) total Smad3. Significance of differences: *p<0.05 vs TGF-β1.