Table 2. Monitoring colicin expression using reporter vectors triggered by colicin extracts.
Function | DNA degradation | RNA degradation | Pore-formation | Control | |||||||
Colicin | ColE2 | ColE7 | ColE6 | ColD | ColA | ColK | ColIb | BZB | |||
Dilution | 3×10−6 | 3×10−3 | 3×10−5 | 3×10−2 | 3×10−2 | 8×10−4 | 4×10−1 | 1×100 | |||
Lum-E2 | 126.9±5.9 | 141.8±2.7 | 1.2±.05 | 42.0±4.4 | 1.5±0.1 | 5.2±0.5 | 9.4±0.8 | 1.1±0.10 | |||
Lum-E7 | 135.9±9.2 | 140.5±2.1 | 1.3±0.2 | 55.5±8.9 | 1.5±0.2 | 2.8±0.3 | 15±3.2 | 1.0±0.07 | |||
Lum-E6 | 67.8±2.2 | 94.3±4.3 | 1.1±0.1 | 36.6±0.4 | 1.3±0.1 | 3.4±0.6 | 11±0.3 | 1.0±0.05 | |||
Lum-D | 107.8±5.4 | 117.5±2.0 | 1.0±0.05 | 48.0±6.0 | 2.1±0.3 | 2.1±0.5 | 9.2±0.4 | 1.0±.050 | |||
Lum-A | 91.9±7.4 | 103.0±4.4 | 1.4±0.35 | 56.6±4.1 | 1.6±0.2 | 2.2±0.3 | 13±1.3 | 1.0±0.02 | |||
Lum-K | 88.2±4.5 | 109.7±5.2 | 1.4±0.5 | 44.0±2.5 | 1.6±0.1 | 3.3±0.5 | 9.0±2.4 | 1.0±0.03 | |||
Lum-Ib | 44.5±7.0 | 42.4±9.0 | 3.5±0.25 | 11.7±1.8 | 1.1±0.2 | 1.7±0.7 | 2.3±0.1 | 0.9±0.30 | |||
Lum-P(–) | 1.8±0. 5 | 1.6±0.7 | 0.9±0.04 | 1.8±0.1 | 1.1±0.1 | 1.6±.04 | 1.3±0.15 | 1.0±0.00 |
Data presented as mean ± standard deviation. Threshold set for induction: RLU/RLU0>2.