Table 3. Example cases of allelic composition drawn from N max = 25, which included population sub-structuring; values calculated for θ, G’st, and Dest_Chao from these sample data.
| Fst | 0 | 1 | 1 | 1 | 1 |
| G’st | 0 | 1 | 1 | 1 | 1 |
| D | 0 | 0.5 | 0.6 | 0.8 | 1 |
| Pop 1 | A/A | A/A | A/A | A/A | A/A |
| A/A | A/A | A/A | A/A | A/A | |
| A/A | A/A | A/A | A/A | A/A | |
| Pop 2 | A/A | A/A | A/A | A/A | B/B |
| A/A | A/A | A/A | A/A | B/B | |
| A/A | A/A | A/A | A/A | B/B | |
| Pop 3 | A/A | A/A | B/B | B/B | C/C |
| A/A | A/A | B/B | B/B | C/C | |
| A/A | A/A | B/B | B/B | C/C | |
| Pop 4 | A/A | B/B | B/B | C/C | D/D |
| A/A | B/B | B/B | C/C | D/D | |
| A/A | B/B | B/B | C/C | D/D |
The fixation of common alleles removes all heterozygosity and results in inflated estimates of genetic differentiation when using F st and G’st as opposed to D.