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. 2013 May 21;8(5):e63767. doi: 10.1371/journal.pone.0063767

Figure 1. Cryptoporus volvatus extract inhibits PRRSV replication.

Figure 1

(A and B) The Cryptoporus volvatus extract blocks PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-α (10 units/µl) or the Cryptoporus volvatus extract at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Cultures treated with normal saline were set up as control (0 mg/ml). Results are from three independent experiments, each of which was in triplicate. (C) Cryptoporus volvatus extract potently inhibits both PRRSV VR2332 and HV replication in PAMs. A similar virus inhibition assay was performed with PAM cells infected with PRRSV strain VR2332 or HV at an MOI of 0.1 in the presence of IFN-α (10 units/µl) or the Cryptoporus volvatus extract at various concentrations. (D) Determination of cytotoxicity of the Cryptoporus volvatus extract by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the Cryptoporus volvatus extract or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.