Figure 1. Stable cell line A549R responses to both IL-6 and PD-180970.

Luciferase (A) and MTT (B) assays of A549R cells treated with PD-180970 (250 nM) and IL-6 (250 ng/ml) respectively. A549R cells were plated in 96-well plates at a density of 1×10⁴ (100 µl/well in DMEM with 10% FBS). Twelve hours later, media were removed and replaced with fresh media in the presence of IL-6 or PD-180970. Bars show the standard deviation (±SD) (three independent repeats, n = 5 in each repeat). ***p<0.001, **p<0.01,*p<0.05, significant relative to vehicle control. NS, no statistical significance. (C) PD-180970 inhibits STAT3 activity in A549R cells. A549R cells were plated and cultured in 100-mm dishes at 70% confluence. Then cells were treated with PD-180970 (250 nM) for 24 h. DMSO was used as control.