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(A–C) Normalized average number of ejections per field of view in the presence of (A) PEG300 (the corresponding osmotic pressure was calculated from PEG300 concentration as described previously (38)), (B) magnesium chloride (the control sample was prepared in 10-fold diluted His1 buffer containing 3.5 mM MgCl2), and (C) sodium chloride (the control sample was prepared in 10-fold diluted His1 buffer containing 50 mM NaCl). Error bars represent 1 SD.
