Figure 8.

Summary of the mechanism underlying phosphorylation-dependent inhibition with respect to nucleosomal DNA. The intramolecular contact of nonphosphorylated AID with BID-HMG is nonspecific and dynamic. This situation allows BID-HMG to transiently bind with the nucleosomal DNA. In contrast, dense phosphorylation expands the AID region by elevating repulsive forces. This results in an increase of the probability of encounter between BID-HMG and pAID, thereby forming a more robust complex between BID-HMG and pAID. AID and BID are symbolically represented by the red and purple strings, respectively. Red and purple spheres indicate negative and positive net charges, respectively. The HMG is denoted by the cyan L-shaped structure. Red open circles labeled P indicate phosphorylation sites.