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. 2013 May 21;104(10):2222–2234. doi: 10.1016/j.bpj.2013.04.007

Table 1.

Summary of the AFM image analysis

Parameter dFACT-WT dFACT-10SA
H1 3.9 ± 0.3 nm 3.9 ± 0.3 nm
H3 1.6 ± 0.3 nm 1.6 ± 0.4 nm
D1–2 15 ± 5 nm 17 ± 5 nm
D2–3 10 ± 3 nm 11 ± 4 nm
D1–3 21 ± 5 nm 23 ± 7 nm
D1–2/(D1–2 + D2–3) 0.59 ± 0.11 0.62 ± 0.11
Number of molecules analyzed 3 3
Number of frames analyzed 5770 4586

Heights and distances are shown by mean ± SD. Units of areas are arbitrary. The accuracy of the HS-AFM measurement for the z-direction (i.e., background noise) was 0.15 nm. The standard deviations of the Gaussian fits were therefore restricted to >0.15 nm. Note that we did not apply Gaussian fittings to the H2 distributions because the results obtained had no statistical relevance.