Table 1.

Summary of the AFM image analysis

Parameter	dFACT-WT	dFACT-10SA
H1	3.9 ± 0.3 nm	3.9 ± 0.3 nm
H3	1.6 ± 0.3 nm	1.6 ± 0.4 nm
D1–2	15 ± 5 nm	17 ± 5 nm
D2–3	10 ± 3 nm	11 ± 4 nm
D1–3	21 ± 5 nm	23 ± 7 nm
D1–2/(D1–2 + D2–3)	0.59 ± 0.11	0.62 ± 0.11
Number of molecules analyzed	3	3
Number of frames analyzed	5770	4586

Heights and distances are shown by mean ± SD. Units of areas are arbitrary. The accuracy of the HS-AFM measurement for the z-direction (i.e., background noise) was 0.15 nm. The standard deviations of the Gaussian fits were therefore restricted to >0.15 nm. Note that we did not apply Gaussian fittings to the H₂ distributions because the results obtained had no statistical relevance.