Figure 4.

(A) The α parameter, the percentage of GQ molecules unfolded and bound by RPA at saturating RPA concentration, is plotted for L2–L5, which have two to five G-tetrad layers, respectively. (B) The α parameter for O1–O5 constructs, which have two to five nucleotides, respectively, in each loop. The α parameter is obtained from the Langmuir binding isotherm analysis, as shown in Figs. 2B and 3B. The red lines are linear fits to the data, which are consistent with both data sets. The O5 data are not included in the fit, as 100% unfolding is already attained at the O4 construct.