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. 2013 May 21;104(10):2295–2306. doi: 10.1016/j.bpj.2013.03.052

Figure 7.

Figure 7

The integrated model allows us to easily assess the contribution of individual eNOS species or simulate the condition where one pathway is modified. (A) Contribution of NO production by different eNOS species. (B) eNOS protein expression with individual transcription-factor activation silenced. Concentrations of the specific transcription factor are fixed at the static level. (C) Normalized concentration of total phosphorylated eNOS with addition of Akt siRNA (left) or dominant-negative Akt (right) 1 h after onset of shear stress. In the Akt siRNA simulation, total Akt concentration was reduced based on the specific silencing efficiency. In the dominant-negative Akt (DN-Akt) simulation, DN-Akt follows the exact kinetics of wild-type Akt except that it loses its catalytic ability to phosphorylate eNOS. The amount of wild-type Akt remains constant, whereas the amount of DN-Akt is 1×, 2×, 5×, and 10× the amount of wild-type Akt.