Figure 1.

Analysis and comparison of the WWTP plasmid metagenomes. (a), Agarose gel separated recovered CCSD pools from WWTP Morges (M) in 2007 and 2009, and WWTP Visp (V) in 2009, compared with regular DNA size standards (L) and the TOL plasmid (L₂). (b), Percent protein coding genes with function prediction of total nonredundant contigs plus singletons annotated uniformly using IMG/M (Markowitz et al., 2008), for a variety of prokaryotic community metagenomes, viral and plasmid metagenomes (indicated with a black arrow). For metagenome accession numbers and for a complete ranking of 291 metagenomes see Supplementary Information, Supplementary Table S3. (c), COG category predictions (percentages of total) for protein coding genes uniformly annotated using IMG/M in the Morges 2007 and Visp 2009 plasmid metagenomes compared with collective plasmids in IMG/M and the full WWTP metagenome from Aalborg (DK) (Albertsen et al., 2012).