Fig. 3.

Time course of Poly I:C-phosphorylation of Stat3, NF-ĸB and up-regulation of TLR2 and IL-6Rα protein expression: a; Western blot showing the kinetics of Stat3 (pStat3-Tyr705) and NF-ĸB (p-NF-ĸB-p65) phosphorylation and TLR2 protein expression in cell-lysates from monolayers of HBE cells without (control) and with poly I:C (10 μg/ml) stimulation at different time points 0.5, 1, 3, 6, 12, 24, 48 h). ß-tubulin was used as the loading control. b Effects of exogenously added IL-6 (lane 1: untreated control; lane 2: 0.5 ng/ml; lane 3: 1 ng/ml; lane 4: 5 ng/ml; lane 5: 10 ng/ml) on Stat3 phosphorylation, with β-tubulin used as a loading control. c; Western blot comparing the expression of IL-6Rα before (lane 1) and after (lane 2) treatment with 10 μg/ml poly I:C. β-tubulin was used as the loading control