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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: Nucleosides Nucleotides Nucleic Acids. 2009 Feb;28(2):67–77. doi: 10.1080/15257770902736426

FIGURE 1.

FIGURE 1

Reverse-phase HPLC profile of crude reaction mixture of d(CGCGCG) with N-hydroxy-1-amino-6-nitropyrene after stirring for 16 h at pH 5.2 in DMF: H2O (1:9). The excess organic reagents were removed by chloroform extraction. The peaks were monitored at 260 nm (panel A) and 450 nm (panel B). Peak 1 shows the unreacted hexamer, whereas peaks 2, 3, and 4 show the adducted hexanucleotide peaks. Peak 2 was identified as d(CGCG*CG) by piperidine cleavage experiment.