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Determination of the molecular weight cut-off (MWCO = 60 kDa, as indicated by an arrow) for alginate microbeads by an inverse size exclusion chromatography from dependence of molecular weight of pullulan standards on their corresponding partition coefficient KSEC. Circles are experimental data and the line is the best fit of data used to determine the MWCO value from its linear parts as indicated by dashed lines.
