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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: Lab Invest. 2012 Jun 18;92(9):1310–1317. doi: 10.1038/labinvest.2012.87

Figure 1. Screening for miRNAs that can potentially target DNAJB6.

Figure 1

(A) Levels of each of the miRNAs, miR-197, miR-424 and miR-632 from human Breast cancer cell lines MCF10AT, MCF10CA1d.cl.1, SUM159 and MDA-MB-231 were compared to immortalized human mammary epithelial cell line MCF10A using real time-quantitive-PCR. The graph is presented in logarithmic scale for Y-axis. Reactions were performed in triplicate and the experiment was performed twice.

(B) mir-424 and miR-632 were transiently expressed in MCF10A. Total protein extract (20μg) was resolved using SDS-PAGE and subjected to western blot analysis for DNAJB6 levels. β-actin level was determined to confirm equal loading.