Figure 1. Screening for miRNAs that can potentially target DNAJB6.
(A) Levels of each of the miRNAs, miR-197, miR-424 and miR-632 from human Breast cancer cell lines MCF10AT, MCF10CA1d.cl.1, SUM159 and MDA-MB-231 were compared to immortalized human mammary epithelial cell line MCF10A using real time-quantitive-PCR. The graph is presented in logarithmic scale for Y-axis. Reactions were performed in triplicate and the experiment was performed twice.
(B) mir-424 and miR-632 were transiently expressed in MCF10A. Total protein extract (20μg) was resolved using SDS-PAGE and subjected to western blot analysis for DNAJB6 levels. β-actin level was determined to confirm equal loading.