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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: Lab Invest. 2012 Jun 18;92(9):1310–1317. doi: 10.1038/labinvest.2012.87

Figure 2. miR-632 targets DNAJB6 for degradation.

Figure 2

(A) pMIR-REPORT-DNAJB6 (containing putative binding site of miR-632 from the ORF of DNAJB6) was co-transfected with miR-632 expression vector, miR-632-pIRES2EGFP or empty vector control. The assay was performed in triplicate and the experiment was performed twice. The luciferase activity readings were normalized with activity from a co-transfected β-gal expressing control. The error bars represent standard error of mean (SEM)

(B) Levels of DNAJB6 and PTCH1 transcript were evaluated from MCF10A cells treated with miR-632-pIRES2EGFP or empty vector control. GAPDH was used as endorse control. The error bars represent standard error of mean (SEM). The reactions were performed in triplicate and the experiment was performed three times.

(C) MDA-MB-231 cells were treated with X-miR-632 or control (50 and 100 nM). Total protein extract (30μg) was resolved using SDS-PAGE and subjected to western blot analysis for DNAJB6 levels. β-actin levels were determined as loading control.