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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: Lab Invest. 2012 Jun 18;92(9):1310–1317. doi: 10.1038/labinvest.2012.87

Figure 5. miR-632 expression enhances the invasive properties.

Figure 5

(A) (I) MCF10AT cells were transiently transfected miR-632-pIRES2EGFP and were allowed to invade through matrigel coated filters for 18 hrs. Invaded cells were visualized using crystal violet, and the cell number was counted and compared to invasion of cells transfected with empty vector control. (II) MCF10AT cells were transiently transfected with empty pIRES2EGFP vector or miR-632-pIRES2EGFP and their growth in 3-D was assessed. Arrows point the invasive outgrowth. Experiments were performed in triplicate and the experiment was performed two times.

(B) (I) MDA-MB-231 cells were treated with X-miR-632 (100 nM) or the control and the invasion assay was performed as described before. (II) MDA-MB-231 cells were transfected with miRNA inhibitor scrambled control clone pEZX-AM01 (control) or anti-miR-632 pIRES2EGFP and their growth in 3-D was assessed. Arrows point the invasive outgrowth.. Experiments were performed in triplicate and the experiment was performed two times.