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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: Nat Cell Biol. 2013 Jan 27;15(2):201–213. doi: 10.1038/ncb2672

Figure 7.

Figure 7

miR-29b knockdown increases the level of expression of its target genes and miR-29b suppresses metastasis by repressing four microenvironmental targets. (a) Relative expression of miR-29b targets in Zip29-knockdown cells by qPCR (n = 5 independently obtained biological samples, *P < 0.05). (b) The 3′UTR reporters of the indicated miR-29b targets were co-transfected with anti-miR-29b or control inhibitor into HEK293T cells. (n =3 independent experiments performed in triplicate, *P <0.05.) (c,d) BALB/c mice were injected i.v. with 4T1 cells ± miR-29b re-expressing ANGPTL4, LOX, MMP9 or VEGF-A, and euthanized at two weeks. Representative fluorescence micrographs of lungs were taken on a dissection microscope (c) and GFP fluorescence intensity was quantified (d). (n =8 independent mice per group, *P < 0.01.) Data are reported as mean±s.e.m. Scale bar, 1 mm.