Skip to main content
. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Cell. 2012 Jul 19;150(3):606–619. doi: 10.1016/j.cell.2012.07.007

Figure 5. NLRP3 Inflammasome Activation by All Gram-Negative Bacteria Requires TRIF-IFNβ-Caspase-11 Axis.

Figure 5

(A–C) IL-1β production by Pam3CSK4-primed C57BL/6 wild-type and Trif−/− (A) or Ifnar1−/− (B) or caspase-11−/− (C) BMDMs stimulated with indicated bacteria for 16 hr.

(D) Cleaved caspase-1 and IL-1β in the supernatants of Pam3CSK4-primed BMDMs stimulated with indicated bacteria for 16 hr.

(E) IL-1β secretion by Pam3CSK4-primed C57BL/6 wild-type and Trif−/− BMDMs stimulated with P. aeruginosa PAK wild-type strain or pscC mutant strain (that lacks the type III secretion system) for 16 hr.

(F) Secreted and cleaved IL-1β in the supernatants of BMDMs primed as indicated and stimulated with cholera toxin B for 16 hr.

Data are presented as the mean ± SEM of one experiment representative of 2–3 experiments. See also Figure S5 and Table S1.