Figure 4.

Hydrated-B[a]P-7,8-dione-dAde adducts detected from HBEC-KT cells. (A) MS³ chromatogram at 0 h. (B) MS³ chromatogram at 24 h. (C) MS² spectrum. (D) MS³ spectrum at 9.54 min. (E) MS³ spectrum at 11.98 min. HBEC-KT cells (∼5 × 10⁶) were treated with B[a]P-7,8-dione (2 μM, 0.2% (v/v) DMSO) in HBSS buffer containing 1 mM sodium pyruvate. The cell pellets were collected at 0 and 24 h. The DNA from cell pellets was extracted and subsequently hydrolyzed before analysis on an ion trap LC-MS/MS.