Figure 3. Loss of Lkb1 Promotes Melanoma Cell Migration and Invasion In Vitro.

(A) Cells of indicated genotypes were subjected to in vitro wound healing or scratch assay. Representative photomicrographs of TKLkb1^L/L cells and TKp53^L/L; p16^L/L are shown on the left. Mean closure index, determined as described in the Supplemental Experimental Procedures, is graphed by genotype on the right (n = 3 replicates per genotype).

(B) Cells of indicated genotypes were subjected to Matrigel invasion assay. Representative photomicrographs of cells that have invaded through Matrigel are shown on the left and mean values graphed for cells of indicated genotypes on the right (n = 3 replicates per genotype).

(C) Western analysis of TKp53^L/L; Lkb1^L/L cells transduced with nonfunctional Lkb1-KD (“kinase dead”) or Lkb1, and TKp53^L/L; p16^L/L cells transduced with nonspecific shRNA (NS) or shRNA targeting Lkb1 (shLkb1) is presented. U, untreated.

(D and E) Isogenic cells with and without Lkb1 as indicated were subjected to in vitro scratch assay (D) and Matrigel invasion assay (E) as in (A) and (B), respectively. Error bars show SD. *p < 0.05; **p < 0.01.

See also Movies S1 and S2, and Figure S3.