Figure 6. Lkb1 Loss Expands a Prometastatic CD24⁺ Cell Population.

(A) CD24 expression of melanoma cells of indicated genotypes was examined by flow cytometry.

(B) TKp53^L/L; Lkb1^L/L cells were transduced with Lkb1-KD or Lkb1. TKp53^L/L; p16^L/L cells were transduced with NS or shLkb1. Cells with and without Lkb1 function as indicated were examined for CD24 expression by flow cytometry.

(C and D) CD24⁺ cells and CD24⁻ cells were isolated from TKp53^L/L; Lkb1^L/L cells by FACS. Sorted cells were subjected to scratch assay (C) and Matrigel invasion assay (D).

(E) CD24⁺ and CD24⁻ cells were isolated from TKp53^L/L; Lkb1^L/L and TKp53^L/L; p16^L/L cells. Cell lysates were subjected to direct immunoblotting or immuno-blotting following immunoprecipitation as indicated.

Error bars show SD. **p < 0.01; ***p < 0.001.

See also Figure S6 and Tables S2 and S3.