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. Author manuscript; available in PMC: 2014 Apr 25.

Published in final edited form as: Chem Biol Interact. 2013 Feb 27;203(2):401–411. doi: 10.1016/j.cbi.2013.02.003

Fig. 1 — Deletion studies showing that Ainp1 interacted with the HLH domain of ARNT. (A) Human ARNT deletion constructs used for this study. (B) Western using anti-Thio mouse IgG to show expression of all thioredoxin (Thio) fusions of ARNT deletions. Arrows indicate the bands of interest. Anti-Ainp1 mouse IgG was used to co-precipitate bacterially expressed Thio-ARNT D1/D2 (C) or Thio-ARNT D1A/B/C (D) or Thio-ARNT basic/HLH (E) using 6His-Ainp1 as bait. Anti-Thio mouse IgG was used to detect Thio-ARNT deletions. Thioredoxin and mouse IgG were used as negative controls. Arrows indicate the bands of interest. Asterisks (*) in C and D indicate the heavy and light chains of mouse IgG of the IP antibodies (anti-Ainp1 mouse IgG) detected by the anti-mouse secondary antibodies. Amount of protein used: +, 40 µg of protein used. These experiments (C–E) were repeated twice with similar results.

Fig. 1 — Deletion studies showing that Ainp1 interacted with the HLH domain of ARNT. (A) Human ARNT deletion constructs used for this study. (B) Western using anti-Thio mouse IgG to show expression of all thioredoxin (Thio) fusions of ARNT deletions. Arrows indicate the bands of interest. Anti-Ainp1 mouse IgG was used to co-precipitate bacterially expressed Thio-ARNT D1/D2 (C) or Thio-ARNT D1A/B/C (D) or Thio-ARNT basic/HLH (E) using 6His-Ainp1 as bait. Anti-Thio mouse IgG was used to detect Thio-ARNT deletions. Thioredoxin and mouse IgG were used as negative controls. Arrows indicate the bands of interest. Asterisks (*) in C and D indicate the heavy and light chains of mouse IgG of the IP antibodies (anti-Ainp1 mouse IgG) detected by the anti-mouse secondary antibodies. Amount of protein used: +, 40 µg of protein used. These experiments (C–E) were repeated twice with similar results.