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. Author manuscript; available in PMC: 2014 Apr 25.

Published in final edited form as: Chem Biol Interact. 2013 Feb 27;203(2):401–411. doi: 10.1016/j.cbi.2013.02.003

Fig. 3 — Timecourse study of protein transduction of 6His-TAT-Ainp1. (A) Western showing the intracellular 6His-TAT-Ainp1 peptide levels within 4 h of 2 µM treatment in HeLa cells. (B) Western showing the timecourse of the intracellular 6His-TAT-Ainp1 or 6His-TAT-ΔGFP peptide levels from 0–144 h. Fresh media was exchanged 2 h after 2 µM treatment. 16% tricine gel was used for analysis [39]. Arrows indicate the bands of interest. Error bars indicate the variations of the means (mean ± SD, n = 3). *** p < 0.001; ns, not significant. The experiments (A and B) were repeated twice with similar results.

Fig. 3 — Timecourse study of protein transduction of 6His-TAT-Ainp1. (A) Western showing the intracellular 6His-TAT-Ainp1 peptide levels within 4 h of 2 µM treatment in HeLa cells. (B) Western showing the timecourse of the intracellular 6His-TAT-Ainp1 or 6His-TAT-ΔGFP peptide levels from 0–144 h. Fresh media was exchanged 2 h after 2 µM treatment. 16% tricine gel was used for analysis [39]. Arrows indicate the bands of interest. Error bars indicate the variations of the means (mean ± SD, n = 3). *** p < 0.001; ns, not significant. The experiments (A and B) were repeated twice with similar results.