. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: Biol Trace Elem Res. 2012 Sep 19;150(0):467–476. doi: 10.1007/s12011-012-9503-9

Table 2.

Assessment of cytotoxicity in HCT-116 cells treated with calcium and gadolinium

	A: Annexin V–propidium iodide			B: Replating

Group	% live	% Dead	% Apoptotic	Number of viable cells (×10⁻⁴)

	5 hours			5 hours	18 hours
SMEM + dFBS	96.2±1.2	2.9±1.0	0.7±0.2	3.2±0.9	3.9±1.3
+1.5 mM calcium	92.0±2.9	4.6±1.2	0.9±0.1	4.6+0.7	6.1±0.6
+50 µM Gd³⁺	92.1±2.6	6.5±1.9	1.0±0.6	4.2±1.1	3.2±0.4^*
+3.0 mM calcium	91.8±1.6	6.6±1.1	1.1±0.9	4.4±1.1	6.1±0.7
+50 µM Gd³⁺	91.6±2.9	7.0±2.8	0.8±0.3	4.9±0.6	2.5±0.7^*

Values shown are means and standard deviations based on duplicate experiments with three samples per data point for each condition in each experiment. Statistical significance of the data in the replating assay was assessed by ANOVA, followed by paired-group comparisons

p<0.05 (statistically significant decrease relative to controls)