Table 2.

Quantitation of Recto-anal Junction Squamous Epithelial (RSE) Cells with Adherent Bacteria in the Absence and Presence of Antisera

	Percent mean±standard error of mean, of RSE cells with adherent bacteria in the ranges showna(MOI=106 bacteria:105RSE cells)
	No antisera			Pooled antisera			Pooled hyperimmune bovine sera
STEC serogroup tested	Bacterial adherence pattern	>10	1–10b	Bacterial adherence pattern	>10	1–10	Bacterial adherence pattern	>10	1–10
O26	Diffuse, moderate	0.7±0.7	86.2±1.7	Diffuse, moderate	32.9±12.9	63.1±8.9	Diffuse, moderate	2±0.6	80±2
O113	Diffuse, moderate	2.0±0.5	93.3±2.5	Aggregative, strong	83±3	12.9±0.9	Aggregative, strong	81.5±3.5	13±2
O103	Diffuse, moderate	0	89.1±4.1	Diffuse, moderate	0	89.8±0.8	Diffuse, moderate	0	78±2
O121	Diffuse, moderate	0	63.3±13.3	Diffuse, moderate	10.3±2.3	83.8±3.8	Diffuse, moderate	0	90±1
O45	Diffuse, moderate	0	65.2±14	Diffuse, moderate	0	92.5±7.5	Diffuse, moderate	0	84.3±0.8
O145	Aggregative, moderate	14.4±13.1	71.9±8.1	Aggregative, strong	68±4	29.5±4.5	Aggregative, strong	82±4	12.45±1.45
O111	Aggregative, strong	72.1±7.1	27.9±7.1	Aggregative, strong	66±4	31.9±5.9	Aggregative, strong	82.4±2.4	16.5±1.5
O157	Aggregative, moderate	0	95.8±4.3	Aggregative, moderate	33±2	66.5±1.5	Non-adherent	0	42.6±4.6

^aAverage of two trials conducted for 2 strains, A and B, of each serotype, except for serotype O121:H19 for which only one strain was available, is shown. Each trial had one slide per bacterial group. Each slide in turn had eight technical replicates spotted on it; 10–20 well-dispersed RSE cells were evaluated per spot. Percent means for ranges used to determine “moderate or strong” adherence, in the absence of any antisera, are shown in bold.

^bNumber of bacteria adhering to each RSE cell is shown as a range of >10, and 1–10. Number of RSE cells without bacteria is not shown.

STEC, Shiga toxin–producing Escherichia coli; MOI, multiplicity of infection.