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. 2013 Apr;10(4):375–381. doi: 10.1089/fpd.2012.1382

Table 3.

Quantitation of HEp-2 Cells with Adherent Bacteria in the Absence and Presence of Antisera

 
Percent mean ± standard error of mean, of HEp-2 cells with adherent bacteria in the ranges showna(MOI = 106 bacteria:105 recto-anal junction squamous epithelial cells)
STEC
No antisera
Pooled antisera
Pooled hyperimmune bovine sera
serogroup tested Bacterial adherence pattern >10 1–10b Bacterial adherence pattern >10 1–10 Bacterial adherence pattern >10 1–10
O26 Diffuse, moderate 1.5 ± 1.5 86.2 ± 13.9 Non-adherent 0.8 ± 0.8 38.2 ± 10.2 Non-adherent 3.57 ± 2.1 27.4 ± 0.4
O113 Diffuse, moderate 0.7 ± 0.7 79.35 ± 4.4 Non-adherent 0 23.7 ± 5.7 Non-adherent 0 17.3 ± 5.6
O103 Diffuse, moderate 0 59 ± 4 Non-adherent 2.1 ± 2.1 42.5 ± 5.9 Non-adherent 0 34.65 ± 5.4
O121 Diffuse, moderate 0 81.2 ± 15.2 Non-adherent 1.4 ± 1.4 40.8 ± 0.8 Non-adherent 0 37.5 ± 7.5
O45 Diffuse, moderate 3.2 ± 3.2 54.3 ± 0.7 Non-adherent 4.2 ± 4.2 18.44 ± 4.9 Non-adherent 5.4 ± 5.4 17.5 ± 7.5
O145 Diffuse, moderate 0 75.5 ± 7.5 Non-adherent 1.5 ± 1.5 43.15 ± 5.2 Non-adherent 3.6 ± 3.6 26.4 ± 11.4
O111 Diffuse, moderate 0 58.5 ± 6.5 Non-adherent 1.3 ± 1.3 17.5 ± 2.5 Non-adherent 1.4 ± 1.4 17.1 ± 2.1
O157 Diffuse, moderate 0 78.3 ± 9.3 Non-adherent 0 26 ± 4 Non-adherent 0 37.6 ± 6.2
a

Average of two trials conducted for two strains, A and B, of each serotype, except for serotype O121:H19 for which only one strain was available, is shown. Each trial had one slide per bacterial group. Each slide in turn had six technical replicates in separate chambers on it; 10–20 well-dispersed HEp-2 cells were evaluated per chamber. Percent means for ranges used to determine “moderate or strong” adherence, in the absence of any antisera, are shown in bold.

b

Number of bacteria adhering to each HEp-2 cell is shown as a range of > 10, and 1–10. Number of HEp-2 cells without bacteria is not shown.

MOI, multiplicity of infection; STEC, Shiga toxin–producing Escherichia coli.