Figure 1. Expression of soluble form of Jagged1 (sJag1) in 3T3-L1 cells. (A) 3T3-L1 cells were transduced with vector control (control) or sJag1 and sorted for GFP expression. (B and C) Cells under low (B) and higher magnification (C) showing the fibroblastic phenotype in sJag1 cells. (D) Phalloidin staining to show altered F-actin organization in sJag1 expressing cells (bar indicates 50 μm). (E) RT-PCR for sJag1 and Notch target gene Hrt-2 transcripts. (F) Immunoblot for endogenous levels of active Notch receptor activation (NICDs, Notch1, Notch2, Notch3 and Notch4) using whole cell lysates. Full-length Jagged1 overexpressing cells were used as positive control to indicate Notch activation. The graph shows quantification of the immunoblot based on integrated densities (graphed are means ± SD).