Figure 3. Characterization of the T-cell phenotype of peripheral blood lymphocytes and tumor-associated lymphocytes from neuroblastoma patients. (A and B) T cells exhibiting the phenotype of naïve, central memory (T_CM), effector memory (T_EM) and CD45RA⁺ effector memory (T_EMRA) cells were identified by flow cytometry. (A) The percentage of naïve, T_CM, T_EM and T_EMRA cells within the CD3⁺CD8⁺ (upper row) and CD3⁺CD8⁻ (lower row) cell populations was determined for the peripheral blood lymphocytes (PBLs, gray bars) and tumor-associated lymphocytes (TALs, black bars) of each patient on the day of tumor removal. (B) Example of CD45RA and CCR7 detection in samples from patient 4.