Table 2.

PicoGreen DNA standard curve

Number on 96 well plate	Stock [DNA] (μg/mL)	Vol. (μL) of DNA standard	Vol. (μL) of working PicoGreen solution	Final [DNA] (μg/mL)
A1, B1, C1, D1	0.0781	20	80	0.0156
A2, B2, C2, D2	0.1563	20	80	0.0313
A3, B3, C3, D3	0.3125	20	80	0.0625
A4, B4, C4, D4	0.625	20	80	0.125
A5, B5, C5, D5	1.25	20	80	0.25
A6, B6, C6, D6	2.5	20	80	0.5
A7, B7, C7, D7	5.0	20	80	1.0
A8, B8, C8, D8 (*)	0	20 uL PBS with 0.1 % Triton-X-100	80	blank

^(*)Represents wells used for PicoGreen background subtraction.