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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Angiogenesis. 2012 Apr 7;15(3):409–420. doi: 10.1007/s10456-012-9269-x

Fig. 1.

Fig. 1

Loss of ARNT inhibits hypoxia enhanced vessel outgrowth and branching in aortic-ring explants. (A) Magnification of vessel outgrowth from rings stained for Rhodamine-CD31 (a, b). Note the vacuolated vessels in b (Arrowhead). Fluorescent micrographs of rings dissected from aortas of ArntloxP/loxP adult mice were infected with Ade -GFP (c) or -Cre/GFP (d). (B) Phase-contrast micrographs of representative rings infected with Ade -GFP or -Cre/GFP after culturing for 10 days under normoxic or hypoxic (2.5% O2) conditions. Arrows indicate vessel branches. Quantification of vascular outgrowth assessed by the number of vessels, length of vessels (C) and branches (D). Data shown are the mean ± SEM, n=10 samples. Statistical analysis was performed using an unpaired Student’s t-test.