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(Left) SFG amide I spectra of interfacial Gβγ (25 μg/mL) adsorbed onto a POPC/POPC bilayer; (Right) Gβγ orientation deduced based on SFG intensity ratio obtain from left spectra. It tilts −35° against the surface normal from the “zero” position defined earlier in ref. 159. Reprinted with permission from ref. 194. (2007 American Chemical Society)
