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Effect of equol and 4-OHT on cytochrome-c expression. MCF-7 were treated for 48 h with equol (100 μM) and 4-OHT (10 μM) and mitochondrial and cytosolic extracts were prepared. Protein expression was then analyzed by western blot. Data are representative of three individual experiments. C, vehicle control; E, Equol (100 μM); T, 4-OHT (10 μM), E + T, Equol (100 μM) + 4-OHT (10 μM).
