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. 2013 Apr 30;8:105. doi: 10.1186/1748-717X-8-105

Figure 2.

Figure 2

Patupilone does not affect MMP transcription. A, MMP mRNA levels in HT1080 cells were determined using qRT-PCR after treatment with 0.2 nM patupilone 24 h prior to IR. B and C, HT1080 cells were treated with 0.2 nM patupilone 24 h prior to treatment with PMA (0.65 mM). mRNA for qRT-PCR (B) and CM for determination of MMP activity (C) were isolated 18 h thereafter, n > 4. The fold increase in mRNA levels and MMP activity was normalized to the mRNA levels and MMP activity from untreated and sham-irradiated cells.*P < 0.05, **P < 0.01, ***P < 0.001.