Table 3.
Polysaccharide content and composition
| Line | Cellulose | Matrix polysaccharides | Rhamnose | Fucose | Arabinose | Xylose | Mannose | Glucose | Galactose | Frequency of [Me]GlcA branching of xylan | % methylated GlcA on xylan |
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT Col-0 |
38.9 ± 2.3 |
35.6 ± 1.4 |
3.4 ± 0.1 |
2.2 ± 0.4 |
12.7 ± 0.4 |
58.7 ± 1.3 |
2.8 ± 0.2 |
5.7 ± 1.7 |
14.5 ± 0.3 |
13.3 ± 0.6 |
64.9 ± 0.6 |
|
pal1-2 |
41.6 ± 5.4 |
35.7 ± 1.8 |
3.0 ± 0.3** |
2.0 ± 0.5 |
13.2 ± 0.4 |
55.2 ± 3.9* |
2.3 ± 0.3* |
9.8 ± 4.8 |
14.5 ± 0.4 |
13.2 ± 0.9 |
66.3 ± 0.8 |
|
pal1-3 |
42.9 ± 4.0 |
37.3 ± 1.1 |
3.3 ± 0.2 |
2.3 ± 0.3 |
12.7 ± 0.7 |
56.0 ± 3.0 |
2.6 ± 0.2 |
8.9 ± 4.2 |
14.2 ± 0.5 |
13.2 ± 1.0 |
66.8 ± 1.0 |
|
pal2-2 |
41.5 ± 4.6 |
37.1 ± 1.5 |
3.5 ± 0.1 |
2.3 ± 0.3 |
12.6 ± 0.8 |
57.3 ± 2.7 |
2.9 ± 0.2 |
7.1 ± 4.3 |
14.4 ± 0.8 |
13.2 ± 0.6 |
64.2 ± 1.0 |
|
pal2-3 |
41.4 ± 2.5 |
36.8 ± 1.7 |
3.4 ± 0.2 |
2.3 ± 0.2 |
12.3 ± 0.8 |
56.7 ± 3.4 |
2.7 ± 0.3 |
8.7 ± 5.1 |
13.9 ± 0.7 |
13.1 ± 0.9 |
64.6 ± 1.1 |
|
c4h-2 |
36.3 ± 2.1 |
42.9 ± 1.7** |
3.6 ± 0.1 |
2.7 ± 0.4* |
15.4 ± 0.9** |
57.0 ± 1.9 |
2.8 ± 0.1 |
3.1 ± 0.2 |
15.4 ± 0.9* |
12.6 ± 0.5 |
80.3 ± 1.2** |
|
c4h-3 |
40.7 ± 3.0 |
38.7 ± 0.9** |
3.3 ± 0.3 |
2.5 ± 0.4 |
14.4 ± 0.9** |
59.0 ± 1.2 |
2.8 ± 0.4 |
2.7 ± 0.6 |
15.5 ± 1.0* |
12.9 ± 0.6 |
72.1 ± 1.5** |
|
4cl1-1 |
41.4 ± 2.6 |
37.8 ± 1.4** |
3.5 ± 0.2 |
2.3 ± 0.5 |
13.3 ± 0.4 |
58.9 ± 1.5 |
2.9 ± 0.2 |
4.3 ± 1.3 |
14.8 ± 0.3 |
13.0 ± 0.4 |
74.7 ± 1.8** |
|
4cl1-2 |
40.7 ± 2.8 |
39.4 ± 1.2** |
3.6 ± 0.1 |
2.2 ± 0.4 |
12.4 ± 0.7 |
58.6 ± 1.6 |
3.0 ± 0.1 |
6.2 ± 1.8 |
14.1 ± 0.6 |
13.1 ± 1.0 |
74.1 ± 1.5** |
|
4cl2-1 |
37.8 ± 2.2 |
38.9 ± 1.4** |
3.7 ± 0.3** |
2.3 ± 0.2 |
12.1 ± 1.0 |
56.7 ± 3.3 |
2.8 ± 1.1 |
8.5 ± 6.5 |
13.8 ± 0.9 |
12.8 ± 0.4 |
64.5 ± 0.6 |
|
4cl2-3 |
38.5 ± 2.3 |
38.3 ± 1.3** |
3.7 ± 0.2** |
2.1 ± 0.4 |
12.0 ± 0.4 |
57.4 ± 2.5 |
3.1 ± 0.2 |
7.6 ± 2.9 |
14.0 ± 0.3 |
12.7 ± 0.6 |
63.8 ± 1.2 |
|
ccoaomt1-3 |
39.8 ± 1.7 |
39.6 ± 1.4** |
3.8 ± 0.2** |
2.4 ± 0.1 |
12.4 ± 0.6 |
59.6 ± 1.2 |
3.2 ± 0.2 |
3.8 ± 0.8 |
14.9 ± 0.4 |
13.4 ± 0.7 |
68.8 ± 2.1** |
|
ccoaomt1-5 |
41.0 ± 2.6 |
38.4 ± 1.4** |
3.4 ± 0.1 |
2.2 ± 0.4 |
13.5 ± 1.4 |
57.1 ± 5.1 |
3.0 ± 0.6 |
5.0 ± 2.9 |
15.8 ± 1.4** |
13.5 ± 0.4 |
73.5 ± 0.6** |
|
ccr1-3 |
23.4 ± 3.5** |
46.6 ± 1.5** |
3.1 ± 0.3 |
1.8 ± 0.4 |
19.2 ± 1.3** |
50.3 ± 2.2** |
2.4 ± 0.3 |
4.9 ± 3.4 |
18.2 ± 1.0** |
14.2 ± 0.4 |
55.2 ± 1.1** |
|
ccr1-6 |
30.9 ± 2.6** |
44.6 ± 1.3** |
3.6 ± 0.3 |
2.2 ± 0.5 |
15.7 ± 0.4** |
54.1 ± 1.9** |
2.7 ± 0.1 |
5.6 ± 2.4 |
16.0 ± 0.5* |
14.5 ± 0.4 |
54.9 ± 1.8** |
|
f5h1-2 |
31.7 ± 2.0** |
35.7 ± 1.3 |
3.6 ± 0.1 |
2.1 ± 0.5 |
12.6 ± 0.4 |
57.0 ± 1.9 |
3.0 ± 0.1 |
7.4 ± 2.2 |
14.4 ± 0.2 |
13.0 ± 0.7 |
66.9 ± 0.6 |
|
f5h1-4 |
32.8 ± 3.2** |
35.8 ± 1.3 |
3.5 ± 0.2 |
2.0 ± 0.4 |
12.5 ± 0.9 |
55.6 ± 3.7 |
2.8 ± 0.3 |
9.2 ± 5.5 |
14.4 ± 0.7 |
13.0 ± 0.3 |
66.3 ± 0.7 |
|
comt-1 |
33.4 ± 1.8** |
37.2 ± 1.7 |
3.4 ± 0.2 |
2.1 ± 0.4 |
12.7 ± 0.7 |
56.5 ± 2.6 |
2.9 ± 0.2 |
7.6 ± 3.6 |
14.8 ± 0.4 |
12.7 ± 0.4 |
65.6 ± 1.9 |
|
comt-4 |
33.2 ± 2.1** |
36.1 ± 0.9 |
3.2 ± 0.1 |
2.2 ± 0.4 |
12.8 ± 0.5 |
55.2 ± 2.6* |
2.8 ± 0.2 |
7.9 ± 3.4 |
15.9 ± 0.5** |
13.1 ± 0.8 |
65.2 ± 0.6 |
|
cad6-1 |
40.6 ± 3.2 |
34.8 ± 1.2 |
3.2 ± 0.1 |
2.3 ± 0.5 |
13.5 ± 0.9 |
58.8 ± 1.6 |
2.6 ± 0.1 |
5.1 ± 2.7 |
14.5 ± 0.5 |
13.0 ± 0.5 |
66.2 ± 0.7 |
| cad6-4 | 38.9 ± 3.3 | 36.2 ± 1.1 | 3.3 ± 0.1 | 2.2 ± 0.3 | 12.7 ± 0.5 | 57.5 ± 1.8 | 2.8 ± 0.1 | 7.1 ± 2.4 | 14.4 ± 0.6 | 13.0 ± 0.7 | 66.4 ± 0.5 |
Cellulose and matrix polysaccharide contents for the 20 lignin mutants (n = 8) and wild-type (n = 16) (± SD) were expressed as percentage CWR. Matrix polysaccharide content was determined gravimetrically based on a TFA extraction and its composition was expressed as mol%. Xylan branching frequency and the percentage methylation of GlcA were determined by DASH. GlcA: glucuronic acid. Increases and decreases compared to the wild-type are indicated in bold and italics, respectively. * 0.001 <p < 0.01; ** p < 0.001 (Dunnett-Hsu adjusted t-test).