Table 4.

Cellulose conversions and pretreatment effect

Line	Conversion without (% cellulose)	Conversion with (% cellulose)	Pretreatment effect (%)
WT Col-0	16.3 ± 2.2	18.1 ± 2.5	11.2 ± 3.3
pal1-2	17.6 ± 2.8	18.5 ± 5.7	5.7 ± 28.0
pal1-3	16.8 ± 2.0	19.2 ± 2.4	14.5 ± 3.7
pal2-2	17.3 ± 2.6	19.1 ± 3.4	10.3 ± 7.1
pal2-3	16.8 ± 1.6	18.1 ± 1.6	8.0 ± 7.0
c4h-2	50.5 ± 4.2**	78.9 ± 5.7**	56.4 ± 6.1**
c4h-3	24.1 ± 2.6**	41.2 ± 3.7**	71.8 ± 9.7**
4cl1-1	21.2 ± 2.1**	30.8 ± 5.1**	44.9 ± 14.7**
4cl1-2	20.5 ± 1.9**	29.2 ± 2.8**	43.0 ± 11.4**
4cl2-1	18.6 ± 2.5	20.3 ± 2.0	9.8 ± 6.8
4cl2-3	17.9 ± 1.0	19.5 ± 1.3	8.9 ± 3.6
ccoaomt1-3	21.8 ± 1.2**	29.8 ± 3.3**	36.1 ± 10.4*
ccoaomt1-5	20.4 ± 4.6*	33.3 ± 5.4**	75.8 ± 75.3**
ccr1-3	77.9 ± 14.6**	88.3 ± 12.8**	14.5 ± 11.1
ccr1-6	64.0 ± 4.8**	76.8 ± 5.6**	20.1 ± 6.1
f5h1-2	22.8 ± 2.4**	23.1 ± 1.8	1.6 ± 3.3
f5h1-4	21.9 ± 2.5**	23.1 ± 2.9	5.8 ± 6.9
comt-1	24.8 ± 0.4**	28.5 ± 1.3**	14.9 ± 4.8
comt-4	25.4 ± 3.0**	28.2 ± 2.3**	11.4 ± 6.1
cad6-1	16.0 ± 1.4	17.9 ± 1.8	11.5 ± 5.2
cad6-4	17.8 ± 1.9	20.1 ± 2.7	13.2 ± 5.2

Cellulose conversions for the 20 lignin mutants (n = 8) and the wild-type (WT) (n = 16) (± SD) without and with acid pretreatment were calculated based on the cellulose content and the saccharification yield (both on a CWR basis) and are expressed as percentage cellulose. The pretreatment effect illustrates the percentage increase in cellulose conversion due to the acid pretreatment compared to cellulose conversion without pretreatment and is calculated as follows: (conversion with – conversion without)/(conversion without) × 100 = %. Increases compared to the wild-type are indicated in bold. * 0.001 <p < 0.01; ** p < 0.001 (Dunnett-Hsu adjusted t-test).