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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: J Mol Cell Cardiol. 2008 Oct 26;46(2):268–277. doi: 10.1016/j.yjmcc.2008.10.008

Figure 2. 2-DE map of cardiac proteins in the mitochondrial and cytosolic fraction.

Figure 2

Subcellular protein extracts were pre-labelled with Cy3 and Cy5 using the DIGE approach and co-separated on large format 2-DE gels using pH 3-10NL IPG strips followed by 12% SDS polyacrylamide gels. Images were acquired on a fluorescence scanner and counterstained with silver. A silver-stained image of mitochondrial extracts and a DIGE image of cytosolic extracts is shown in panel A and B, respectively. Analyses using DeCyder® software revealed the spots showing a significant difference in AE or DN hearts. Proteins were numbered and identified by nano-LC MS/MS (Supplemental Table I–III).