Table 2. eat-4 mutants recorded with NeuroChip or conventional microelectrode recordings have the same phenotype.
Conventional recording | NeuroChip | |
Eat-4(n2474) (n = 5) | ||
EPG frequency (s−1) | 0.055±0.007 | 0.090±0.015 |
EPG duration (s) | 0.287±0.014****1 | 0.172±0.009****3 |
Average number of P waves | 0.280±0.065*2 | 0.103±0.022****4 |
‘e’ amplitude (mV) | 0.368±0.030 | 0.376±0.034 |
R-E interval (s) | 26.513±3.078 | 22.288±3.867 |
R-E ratio | 2.170±0.117 | 1.491±0.085 |
Data are the mean ± s.e.mean of recordings from ‘n’ worms. Each parameter was derived from AutoEPG analysis of all the EPGs captured in a recording of 5 minutes or, for manual analysis, from 60 EPGs. Eat-4 had increased pump duration and decreased number of ‘P’ waves compared to wild-type. Comparisons made by unpaired Student’s t-test to the respective parameters for 1,2 conventional wild-type N2 recording without 5-HT, P<0.0001 and P = 0.0208, respectively; or 3,4 to wild-type N2 NeuroChip recording without 5-HT, P<0.0001. Wild-type data are provided in Table 1.