Figure 3. SPT of individual QDs non-specifically adhered to a glass surface.

(A) Progressing from blue to red are sum projections of raw data (solid) and Gaussian fits (dashed) for single 525, 565, 585, 605, 625, 655, 705 and 800 nm QDs. (B) 3D representation of a single hyperspectral time frame with 3D boxes representing sub-volumes identified for further particle localization. Sum projections of the data onto each plane are used to represent the raw data (gray scale). Likewise, 2D projections of each 3D box onto each plane are used to highlight the sub-volumes. Fitting results for the colored sub-volumes (color corresponds to the spectral center of the box) in (B) are shown in (C), (D), and (E). In the top figures in (C), (D), and (E) red and green are sum projections of the raw data and fit respectively. Note that the color outlining (C), (D), and (E) correlate with the respective sub-volumes highlighted in (B) with the same color. The localized particles are represented by white ellipsoids in which the radius in each dimension shows 3 standard deviations in the estimated error in the fit using the Cramér Rao Bound and their projection onto each axis is represented by a gray ellipse. The bottom figures in (C), (D), and (E) show raw spectral features (black) and Gaussian fits (color corresponds to fit spectral emission peak). The vertical gray lines represent the spectral cutoffs for independently fit sub-volumes. See Video S1.