Table 1.
HPLC assay conditions and retention times for the various ester prodrugs.
| Prodrug | Composition of aqueous phase (pH 2.5) | Composition of organic phase | Mobile phase Aq:Org | Retention timesa | ||
|---|---|---|---|---|---|---|
|
| ||||||
| Parent drug | Intermediate | Prodrugb min | ||||
| ACV | 25 mM KH2PO4 | Acetonitrile | 99:1 | 13.7 | – | – |
| AACV | 25 mM KH2PO4 | Acetonitrile | 99:1 | 13.7 | – | 11.6 |
| SSACV | 25 mM KH2PO4 | Acetonitrile | 94:6 | 4.8 | 25.8 | 8.1 |
| SACV | 25 mM KH2PO4 | Acetonitrile | 99:1 | 13.7 | – | 7.3 |
| CACV | 25 mM KH2PO4 | Acetonitrile | 97:3 | 7.6 | – | 19.7 |
Notes:
a
UV detection at λmax = 254 nm;
b
Retention times noted when separately injected as a pure compound onto the column.