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. 2010 Jul 29;2:43–56.

Table 1.

HPLC assay conditions and retention times for the various ester prodrugs.

Prodrug Composition of aqueous phase (pH 2.5) Composition of organic phase Mobile phase Aq:Org Retention timesa

Parent drug Intermediate Prodrugb min
ACV 25 mM KH2PO4 Acetonitrile 99:1 13.7
AACV 25 mM KH2PO4 Acetonitrile 99:1 13.7 11.6
SSACV 25 mM KH2PO4 Acetonitrile 94:6 4.8 25.8 8.1
SACV 25 mM KH2PO4 Acetonitrile 99:1 13.7 7.3
CACV 25 mM KH2PO4 Acetonitrile 97:3 7.6 19.7

Notes:

a

UV detection at λmax = 254 nm;

b

Retention times noted when separately injected as a pure compound onto the column.