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. 2010 Jul 29;2:43–56.

Table 2.

First order hydrolysis rate constants of AACV, SSACV, SACV and CACV in buffers at pH 7.4, 6.0 and 5.0 and in cornea (protein content 0.25 mg/ml).

Buffer AACV SSACV SACV CACV




Rate (k* 104 min−1) Half life (hr) Rate (k* 104 min−1) Half life (hr) Rate (k* 104 min−1) Half life (hr) Rate (k* 104 min−1) Half life (hr)
DPBS 5.0 0.5 ± 0.2 273 ± 148 a 0.4 ± 0.2 353 ± 199 7.5 ± 0.9 15.5 ± 1.7
DPBS 6.0 2.5 ± 0.2 47 ± 5 0.11 ± 0.03 1157 ± 369 0.5 ± 0.1 226 ± 52 17.4 ± 1.6 6.7 ± 0.6
DPBS 7.4 39 ± 1 2.9 ± 0.1 3.2 ± 1 38 ± 12 3.9 ± 0.3 29 ± 3 37.1 ± 3.4 3.1 ± 0.3
Cornea b 83 ± 8 3.5 ± 0.4 12.5 ± 0.8 9.2 ± 0.6 64.4 ± 5.5 1.8 ± 0.1

Notes: Cornea—Corneal hydrolysis in DPBS 7.4. Values are mean ± S.D. (n = 3).

a

No measurable degradation during the period of a 96 hr experiment;

b

Complete degradation before 5 min period.