Table 2.
First order hydrolysis rate constants of AACV, SSACV, SACV and CACV in buffers at pH 7.4, 6.0 and 5.0 and in cornea (protein content 0.25 mg/ml).
| Buffer | AACV | SSACV | SACV | CACV | ||||
|---|---|---|---|---|---|---|---|---|
|
|
|
|
|
|||||
| Rate (k* 104 min−1) | Half life (hr) | Rate (k* 104 min−1) | Half life (hr) | Rate (k* 104 min−1) | Half life (hr) | Rate (k* 104 min−1) | Half life (hr) | |
| DPBS 5.0 | 0.5 ± 0.2 | 273 ± 148 | a | – | 0.4 ± 0.2 | 353 ± 199 | 7.5 ± 0.9 | 15.5 ± 1.7 |
| DPBS 6.0 | 2.5 ± 0.2 | 47 ± 5 | 0.11 ± 0.03 | 1157 ± 369 | 0.5 ± 0.1 | 226 ± 52 | 17.4 ± 1.6 | 6.7 ± 0.6 |
| DPBS 7.4 | 39 ± 1 | 2.9 ± 0.1 | 3.2 ± 1 | 38 ± 12 | 3.9 ± 0.3 | 29 ± 3 | 37.1 ± 3.4 | 3.1 ± 0.3 |
| Cornea | b | – | 83 ± 8 | 3.5 ± 0.4 | 12.5 ± 0.8 | 9.2 ± 0.6 | 64.4 ± 5.5 | 1.8 ± 0.1 |
Notes: Cornea—Corneal hydrolysis in DPBS 7.4. Values are mean ± S.D. (n = 3).
a
No measurable degradation during the period of a 96 hr experiment;
b
Complete degradation before 5 min period.