Figure 4. Analysis of KIR3D protein expression on rhesus macaque T cell subsets.
(A) CD8 αβ T cells were analysed for frequency of KIR expression (left panel), mean fluorescence intensity (antibody 1C7) (middle panel), linear regression analysis of KIR frequency and MFI (right panel). (B) γδ T cells were analysed for frequency of KIR expression (left panel), mean fluorescence intensity (antibody 1C7) (middle panel), linear regression analysis of KIR frequency and MFI (right panel). (C) CD4 αβ T cells were analysed for frequency of KIR expression (left panel), mean fluorescence intensity (antibody 1C7) (middle panel), linear regression analysis of KIR frequency and MFI (right panel). Antibody 2H5 detects KIR3DL05, whereas antibody 2H9 detects KIR3DS05 and KIR3DLW03 in animal 2425 and only KIR3DS05 in animals 2136, 2146, 2405, 14225, and 14218, and only KIR3DLW03 in animal 14229 according to KIR genotyping.
