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. 2013 May 22;8(5):e64558. doi: 10.1371/journal.pone.0064558

Figure 1. Circulating miRNA levels in controls, CKD patients and hemodialysis patients in freshly isolated samples.

Figure 1

Sera were collected from stage 3–4 CKD patients (n = 10), hemodialysis patients (n = 10) and healthy volunteers (n = 8) and total RNA isolated and real time PCR performed to determine the expression of circulating levels of miR-125b (A), miR-145 (B) and miR-155 (C) normalized by U6. Total serum miRNA concentration in the three groups was measured using Agilent Bioanalyzer (D) to demonstrate that the proportion of miRNA to total RNA analyzed is not the etiology of decreased expression of these specific miRNA. Each sample was assayed in triplicate. Data were expressed as mean ± SEM. * p<0.01 compared to healthy volunteers; ** p<0.05 compared to healthy volunteers.